Photomedicine and Laser Surgery
Effect of Low-Level Laser Therapy on Inflammatory Reactions during Wound Healing: Comparison with Meloxicam

To cite this paper: Vinicius Nery Viegas, Marcelo Emir Requia Abreu, Christian Viezzer, Denise Cantarelli Machado, Manoel Sant'Anna Filho, Daniela Nascimento Silva, Rogerio Miranda Pagnoncelli. Photomedicine and Laser Surgery. December 1, 2007, 25(6): 467-473. doi:10.1089/pho.2007.1098.

Vinicius Nery Viegas, M.S. School of Dentistry, Laser Center, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
Marcelo Emir Requia Abreu, M.S. School of Dentistry, Laser Center, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
Christian Viezzer Biomedical Researches Institute, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
Denise Cantarelli Machado, Ph.D. Biomedical Researches Institute, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
Manoel Sant'Anna Filho, Ph.D. School of Dentistry, Oral and Maxillofacial Surgery Department, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
Daniela Nascimento Silva, Ph.D. School of Dentistry, Oral and Maxillofacial Surgery Department, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
Rogério Miranda Pagnoncelli, Ph.D. School of Dentistry, Laser Center, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.

Objective: This study evaluated the action of low-level laser therapy (LLLT) on the modulation of inflammatory reactions during wound healing in comparison with meloxicam.
Background Data: LLLT has been recommended for the postoperative period because of its ability to speed healing of wounds. However, data in the literature are in disagreement about its anti-inflammatory action.
Methods: Standardized circular wounds were made on the backs of 64 Wistar rats. The animals were divided into four groups according to the selected postoperative therapy: group A–control; group B–administration of meloxicam; and groups C and D–irradiation with red (λ = 685 nm) and infrared (λ = 830 nm) laser energy, respectively. The animals were killed at 12, 36, and 72 h and 7 days after the procedure.
Results: Microscopic analysis revealed significant vascular activation of irradiated sites in the first 36 h. Only group B showed decreases in the intensity of polymorphonuclear infiltrates and edema. Group D showed a higher degree of organization and maturation of collagen fibers than the other groups at 72 h. The animals in group C showed the best healing pattern at 7 days. The anti-inflammatory action of meloxicam was confirmed by the results obtained in this research. The quantification of interleukin-1β (IL-1β) mRNA by real-time polymerase chain reaction (PCR) did not show any reduction in the inflammatory process in the irradiated groups when compared to the other groups.
Conclusions: LLLT improves the quality of histologic repair and is useful during wound healing. However, with the methods used in this study the laser energy did not minimize tissue inflammatory reactions.